Device
Part:BBa_K2825001:Design
Designed by: Daniel Zheng Group: iGEM18_UMaryland (2018-10-10)
PETase-CBD-HLYA
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1550
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1489
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1324
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 1306
Design Notes
Hlya tag was added since there is a stop codon in the scar site if we were to assemble the part to iGEM13_TecMonterrey secretion system
Source
Sequence was obtained from Joo,